Yes, Pro-Line recommends that all tires with closed cell and regular foam inserts (including Pre-Mounts) have 2 holes in the tread to prevent air from being trapped in the tire.
Watch how to Fix your Premounted Tire Wobble
The color dot is something that Pro-Line has added to the sidewall of all our tires. This identifies the compound of the tire.
Make sure you use a Cyanoacrylate (CA) type glue.
Check out the Tire Selector Tool!